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The following includes the key steps to address the hurdles and validate the use of nanotechnology: Preclinical research Nanoparticle-enhanced drug delivery and imaging techniques help in the initial validation, which often begins with in vitro (cell culture) experiments to assess the nanomaterials biological interactions, cytotoxicity, drug release profiles, and targeting abilities, mostly done in preclinical research through in vitro studies and using animal models [122, 123]